Enzymatically-active nanoparticles to direct the self-assembly of peptides in hydrogel with a 3D spatial control

Hydroxypropylmethylcellulose grafted with silanol groups (Si-HPMC) hydrogels are employed as 3D scaffolds to encapsulate phosphatase-modified silica nanoparticles ([email protected]). Such hybrid materials catalytically active when enzyme susbtrates diffusing through the material. Using enzyme-assisted self-assembly concept, tripeptide Fmoc-FFY is generated in situ close vicinity of [email protected] thanks phosphate hydrolysis precursor Fmoc-FFpY. The spatially concomitant presence In Si-HPMC host hydrogel, aggregate and thus limit their diffusion This feature used localize at micrometre scale within material: injected a defined area gel using an adapted microsyringe. Fmoc-FFpY toward this region leads exclusively there, impossible strategy free AP. Thus, work allows control peptides alternative approach for design hierarchically structured materials.